Insertion junction: CLIP2.040609_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.040609
Chromosome:chromosome 9
Location:4684125
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401997 (1 of 1) 1.1.1.60 - 2-hydroxy-3-oxopropionate reductase / Tartronate semialdehyde reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGGCGTCGACCTCAGGGCCTGCAGTTGCGCAAGCAGCTCCGACACAG
Internal bar code:CCGTGTTTCTAGTCGTGTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:992
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCCCATGGAAGCAGGTA
Suggested primer 2:ATGGCGCCGTGGATAATGAT