Insertion junction: CLIP2.040631_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040631
Chromosome:chromosome 1
Location:4121048
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g027350 TMG10 (1 of 2) PTHR12029:SF11 - METHYLTRANSFERASE TARBP1-RELATED; tRNA (guanosine-2'-O)-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTTTCCATACACACACGCACACAAATCGCTTCATATCCCATGTTCAA
Internal bar code:CAAAGGGTATCCTCCACCTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:768
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGCTTACACTCCCCACC
Suggested primer 2:CCATAACACGGACTGCCACT