| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040643 |
| Chromosome: | chromosome 1 |
| Location: | 3255111 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020887 | (1 of 2) K06972 - uncharacterized protein (K06972) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGATTCGTGTGGAGGGTGTGTGGGGGAACCGCGGCTCTGTCGGTACG |
| Internal bar code: | ATTCGTCAGGCGGGTGCGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 83.5616 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCATTGCACGTGTCGAGA |
| Suggested primer 2: | TGCAACCCCTGTTAGCTCTC |