Insertion junction: CLIP2.040646_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.040646
Chromosome:chromosome 5
Location:2970956
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g230700 (1 of 274) IPR020683 - Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCTCATCTCACCCGCAGGCCTCCTCCTCCGGCAGCTTCCAGTTGCGC
Internal bar code:CCACCAACCGACTATGATCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:6386
LEAP-Seq percent confirming:98.9691
LEAP-Seq n confirming:96
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:97

Suggested primers for confirmation by PCR

Suggested primer 1:GGGAGGACGACGACTTTGTT
Suggested primer 2:GGCCCTTCTGTCCTTCAGTC