| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040655 |
| Chromosome: | chromosome 16 |
| Location: | 7175999 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676533 | (1 of 1) IPR000742//IPR001599 - EGF-like domain // Alpha-2-macroglobulin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCAGCGGCCCGGACAGGGCGTGGTACCGCGCCCCGCGGGGGAGGCGC |
| Internal bar code: | TCTCGGTTCCAAGGGTTGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2488 |
| LEAP-Seq percent confirming: | 83.1579 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 95 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGTACGGACGCGGTCTT |
| Suggested primer 2: | TGTGATGAAGAACCCGGAGC |