Insertion junction: CLIP2.040663_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.040663
Chromosome:chromosome 7
Location:4927227
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346698 PHC75 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCGGCGCATCCGGCGGCGGCGGTGGGGGTGGATCGGGGGGAGGAGG
Internal bar code:ATGGGAGTCTAGACGGCGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:256
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCCCATCAACACAAACAC
Suggested primer 2:GAAGGAGGAGAAGGCGAAGG