Insertion junction: CLIP2.040663_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.040663
Chromosome:chromosome 7
Location:4927249
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346698 PHC75 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Putative pherophorin-chlamydomonas homolog CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTAGCCCCCCACCGCCCAGCCCTCCGCCTCCCAGCCCGCCTCCTCCTGA
Internal bar code:ATGGGAGTCTAGACGGCGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:77
LEAP-Seq percent confirming:3.44828
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGAGGAGAAGGCGAAGG
Suggested primer 2:GCCCCCATCAACACAAACAC