Insertion junction: CLIP2.040678_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.040678
Chromosome:chromosome 17
Location:3278482
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g722000 MFT30,SOC1 Major facilitator superfamily transporter; (1 of 1) K08214 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 18 (SLC22A18) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCAAGCGCGGTGCCGCGCTGCTGCGGCAGGACGGCCTGCATGTAACAG
Internal bar code:GCTGTAGAACTAATGGTCTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3057
LEAP-Seq percent confirming:96.4912
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTTGCGGACACTCAACTC
Suggested primer 2:AGCCGTGCAACACCAAAATC