Insertion junction: CLIP2.040693_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.040693
Chromosome:chromosome 16
Location:3588300
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g670261 (1 of 1) K15305 - vacuole morphology and inheritance protein 14 (VAC14, TAX1BP2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATCAGCTCCATTGACATGGCGGCCATCCTGGGGACCGTCATCCTGGAA
Internal bar code:CCCTCGCAGCACTGTGTTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2738
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCAATCACAACACAAGCA
Suggested primer 2:GTGGTCTCAGTGGACAGTCG