Insertion junction: CLIP2.040706_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.040706
Chromosome:chromosome 6
Location:3857879
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278182 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGGAGGCAGGGTGGGGCTTAGACATGGTCTTGCAACGATAGCGGCGC
Internal bar code:GCACGTATCCGGCTTCCTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:263
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTATCGAAGCGCCTTTTG
Suggested primer 2:CGGAAGGACACACATGGACA