Insertion junction: CLIP2.040709_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.040709
Chromosome:chromosome 10
Location:5368223
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g456950 CEP10 Cysteine endopeptidase; (1 of 1) IPR000668//IPR008979//IPR013128//IPR018392//IPR029062 - Peptidase C1A, papain C-terminal // Galactose-binding domain-like // Peptidase C1A // LysM domain // Class I glutamine amidotransferase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACACGCACATCGTACACACCTTTCCCCTTGCAAGCTCGCTAAGAAAC
Internal bar code:CTTTTTTTTAGATTTAACCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:405
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAGGACAACATGCGGAAG
Suggested primer 2:GGCTTTGAGGACTCCAACGA