Insertion junction: CLIP2.040713_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.040713
Chromosome:chromosome 17
Location:2344020
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g713700 IAD2 Indoleamine 2%252C3-dioxygenase; (1 of 1) 1.13.11.52 - Indoleamine 2,3-dioxygenase / Tryptophan pyrrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATTACGGCCCCCCTTTCCCCGACCGGCCGCCGCGCGTGCTGTGCGTGC
Internal bar code:AATCATGATGTTCATACTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1996
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGCTCATACACACACAGG
Suggested primer 2:ACCCCATACCTCTCAGGACC