| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040723 |
| Chromosome: | chromosome 16 |
| Location: | 7326528 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678773 | BSD2,BHSD2 | (1 of 1) PTHR10366//PTHR10366:SF355 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // SUBFAMILY NOT NAMED; 3-beta hydroxysteroid dehydrogenase | intron |
| lncRNA_TCONS_00188791 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCACAAAAAGCACCGCATGAAGAAAGGTTACGGTACGCACGACTGG |
| Internal bar code: | TCTTACGTTCTTAGAATACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1150 |
| LEAP-Seq percent confirming: | 2.04082 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATATGGCGGGATGTGTG |
| Suggested primer 2: | CCGCGTTGTACATGTGCAAA |