Insertion junction: CLIP2.040728_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040728
Chromosome:chromosome 9
Location:3843456
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801034 (1 of 1) K08736 - DNA mismatch repair protein MSH3 (MSH3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCTGGAGTAAGCTAACCTGCACCACCACCACCACCACCACCACCACCA
Internal bar code:GTCGGATTGCAGTCCGCCATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2180
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAACCCTCCTCCCACTC
Suggested primer 2:GTGTATAGACGCCGTCAGGG