Insertion junction: CLIP2.040754_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040754
Chromosome:chromosome 3
Location:199463
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144164 RSE2,RSEP2 (1 of 1) PTHR22939:SF79 - PEPTIDASE M50 FAMILY PROTEIN; Intramembrane metalloprotease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGCAGTGCGAGCCGCGCGCGGACCCCGTCACCCAACTAGTTGCAAAT
Internal bar code:ACGTGCGAGTCGTGGCCCCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:355
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACACACG
Suggested primer 2:CACAACTGCACGTCCTACCT