Insertion junction: CLIP2.040754_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040754
Chromosome:chromosome 3
Location:199604
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144164 RSE2,RSEP2 (1 of 1) PTHR22939:SF79 - PEPTIDASE M50 FAMILY PROTEIN; Intramembrane metalloprotease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTTAACCCCTCCGCTGCCCTTCCCCCCACCTGAGCCAGCAGCACTAGG
Internal bar code:TGAATTGACGGGCTTGACGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:231
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CACAACTGCACGTCCTACCT
Suggested primer 2:CACACACACACACACACACG