| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040755 |
| Chromosome: | chromosome 4 |
| Location: | 84810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214657 | (1 of 5) K03549 - KUP system potassium uptake protein (kup) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGAGCGGAGCAGCAGGCGGCAGCAGCAGCAGCGGCAGCAGCAGCAG |
| Internal bar code: | CCCGTGAGAGGATCCTAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 64.7059 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCAGAAACCCCTTCCCC |
| Suggested primer 2: | CTCCGTGTCACTGGCAGTAG |