Insertion junction: CLIP2.040772_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.040772
Chromosome:chromosome 3
Location:1906034
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g154950 GST6 Glutathione S-transferase; (1 of 3) K07393 - putative glutathione S-transferase (ECM4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCCACAGCACAGGCACCGAGAACGTCACTACAGAACAGTGGCAATAT
Internal bar code:TAACAAATACTTGTTGGATCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:618
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCACACATGCAGGGTCTG
Suggested primer 2:CATACCGCTCCCCTCATGTC