Insertion junction: CLIP2.040777_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040777
Chromosome:chromosome 6
Location:3711617
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278157 (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCGCGCCCATGCCCGGGGGGCCGTACAAGGCAGGGTCTGCGCGGCTG
Internal bar code:ACTCATTGAGTGCGTAAACCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3437
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCTGTGCTATTCTGCGC
Suggested primer 2:CTTCTCACCAGCTCCACCTG