| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040779 |
| Chromosome: | chromosome 1 |
| Location: | 4121358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027350 | TMG10 | (1 of 2) PTHR12029:SF11 - METHYLTRANSFERASE TARBP1-RELATED; tRNA (guanosine-2'-O)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGCCTGTCCTCCCGCCAGCAGCTCCAGCGCCAGGCGCCACACGTCA |
| Internal bar code: | TGTACTCTATTGAACGCTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTTTGATATGCGCCGGC |
| Suggested primer 2: | GCGTGGGTATACGTACGTGT |