Insertion junction: CLIP2.040788_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.040788
Chromosome:chromosome 2
Location:2002720
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g088350 SNR3 (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc); SNARE associated Golgi protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACTTACACACACGCCTACGTACACACAGCGCTTGTGCAGATCATTTG
Internal bar code:GATAAGCTTCACGTCAGGTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACGGTGGAGAACTTCGG
Suggested primer 2:GCTAGCGAACAGGCCATTTC