Insertion junction: CLIP2.040818_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040818
Chromosome:chromosome 10
Location:310819
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g419950 (1 of 5) PF00536 - SAM domain (Sterile alpha motif) (SAM_1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACACACACACACACACACACACACACACACACACACAC
Internal bar code:ATTTTTGTCGCGCTGTACTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3047
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAGCGGTCCATATGCAG
Suggested primer 2:ATCTCGGACATGATCGTGGC