Insertion junction: CLIP2.040857_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040857
Chromosome:chromosome 16
Location:2985915
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663750 (1 of 10) PF13519 - von Willebrand factor type A domain (VWA_2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTTGTCGTACAGGGCCAGCCCCGCGTACGACAGAATCTGGGAGGTGG
Internal bar code:TTCACTACAGACCATGCGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4913
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:CACATCCTACCTGGTGAGGC
Suggested primer 2:CATGCATCTTGAGCACTCGC