| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040859 |
| Chromosome: | chromosome 1 |
| Location: | 3124258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019900 | FAP384 | Flagellar Associated Protein 384 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACGTCCTCGAACCTGTGAGGAGGGGCAGTCGCAGCGAAGGGTGGGGG |
| Internal bar code: | TCCTTACGATGACATCAGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1347 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGCATCAAGGTGTCTAT |
| Suggested primer 2: | GCGTAATCGAGGAGCATGGA |