| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040864 |
| Chromosome: | chromosome 13 |
| Location: | 3482396 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587500 | AOF8 | (1 of 2) 1.3.5.5 - 15-cis-phytoene desaturase / Plant-type phytoene desaturase; Flavin-containing amine oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAAGATGAGCGCATACGGTCAGTGCTGACAGCAGGGGGGCGACAGTTG |
| Internal bar code: | ATATAGATGCGTTAGACGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1449 |
| LEAP-Seq percent confirming: | 62.7907 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAAAACTTGAAGTCCGC |
| Suggested primer 2: | GCCACTCTCCCTACTCCTCA |