| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040873 |
| Chromosome: | chromosome 16 |
| Location: | 7492366 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682026 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGGACCGCTCCTTGTCGCTTGAGTCGTGGAACCGGCAGATGCGGAGT |
| Internal bar code: | TAGTCGGTTCCAGACGAATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 854 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCATCCTCATCAGCCGTC |
| Suggested primer 2: | AGAAGGTGAAGCTGCTGCAA |