Insertion junction: CLIP2.040877_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.040877
Chromosome:chromosome 12
Location:3473419
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g497250 CEP95 Centrosomal Protein 95; (1 of 1) IPR026619 - Centrosomal protein of 95kDa CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAGAGGGCTGGGAGCATGCTTTTAACGGCCACGGCGATGGAGAGGGAG
Internal bar code:GTCGGTCGTTCCTAACGATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:456
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGGTGTTGGTCGTAGAAA
Suggested primer 2:TAGTCCCCAGTGCTAGTCCC