| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040902 |
| Chromosome: | chromosome 7 |
| Location: | 1455804 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323750 | HAS2 | (1 of 1) K06133 - 4'-phosphopantetheinyl transferase (LYS5, acpT); Holo-[acyl-carrier-protein] synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTAGAAAGTGCGATATGTGGCGCCTCAAGTGCCGCGTGGTACCAAACG |
| Internal bar code: | CGCGGCAACGCTCATTTGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3475 |
| LEAP-Seq percent confirming: | 92.5373 |
| LEAP-Seq n confirming: | 124 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCAGTGTTGCCTTGAA |
| Suggested primer 2: | CGGCTAGTTATTGTGCGTGC |