| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040908 |
| Chromosome: | chromosome 2 |
| Location: | 1427273 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083450 | (1 of 1) IPR000104//IPR002938 - Antifreeze protein, type I // FAD-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGGATGCGGCAGTGCTGGGCGCGTGCGTGCGGAAGCACGGCCTGGGG |
| Internal bar code: | CGTGGTCCAGAGGGTGATGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4895 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACTTCCAGCACCCTCAG |
| Suggested primer 2: | TCTGCGTCTCTGTGTGTGTC |