| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.040912 |
| Chromosome: | chromosome 6 |
| Location: | 2591479 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269865 | SMM18 | (1 of 1) IPR000104//IPR029044 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferases; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACACACACACACACACACACACCGCCCCGCACAGGCCCGC |
| Internal bar code: | AAAGCTTTGGCACGCTGCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1645 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCTCCTCCTGCTTCC |
| Suggested primer 2: | ACTTAGTGGGCGGGATTTGG |