| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040915 |
| Chromosome: | chromosome 10 |
| Location: | 867309 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423600 | (1 of 1) PTHR10891//PTHR10891:SF5//PTHR22847 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED // WD40 REPEAT PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGCCGCATGAGGGGAGTACCTAGCGTGTGCCGCCTCCGTCACACCA |
| Internal bar code: | TTTTGAAGGAGACCCTCCGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5063 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | CACGTTTGTGCAGGTGTGAG |