| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040942 |
| Chromosome: | chromosome 9 |
| Location: | 3421232 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395769 | FAP177 | Flagellar Associated Protein 177 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGTCAAGGTCGTGCGTGGCCGCCTTCAAGCGGCAGCGTTTACTAGG |
| Internal bar code: | GAGCCTGGTTATCACATTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2066 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGATGCAGCCAACGAGT |
| Suggested primer 2: | ATGCGAGGCAAGGAAGGAAA |