| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.040974 |
| Chromosome: | chromosome 17 |
| Location: | 2587121 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716301 | SUM7 | Similar to Small ubiquitin-like modifier; (1 of 4) PF03171//PF11976 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy) // Ubiquitin-2 like Rad60 SUMO-like (Rad60-SLD) | CDS |
| Cre17.g716350 | GT90F32,GT90-32 | GT90 family protein 32; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTCCGGCCCGGCGCGCCCGCGTGGGGCTCGGGCCGCTCTCGCACGCA |
| Internal bar code: | GGAACGGTATGTATGTATGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4425 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAACTGTACGGTAAGCA |
| Suggested primer 2: | GGACTGGCACAAGCAACAAG |