Insertion junction: CLIP2.040975_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.040975
Chromosome:chromosome 16
Location:6585653
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g674000 WDR2,LWD1 (1 of 1) K11805 - WD repeat-containing protein 68 (WDR68, HAN11); WD-4 repeat family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACATATACACACACACACACATACATATACCCAGCCGC
Internal bar code:AACGTCGCAGTAGCACGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:506
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGAGGTGTACGACATAGC
Suggested primer 2:GGTAGCGGATGTCCAGGATG