Insertion junction: CLIP2.040988_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040988
Chromosome:chromosome 2
Location:6660482
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g390000 SRH17 SNF2-related DNA/RNA helicase; (1 of 2) K11654 - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 [EC:3.6.4.-] (SMARCA5, SNF2H, ISWI) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCTCCGTTGGCCTCCGCTGCCGCGGCGGGCTCCTGCCCCACATCCTG
Internal bar code:TGTAGGAAAGCGATACTCACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1269
LEAP-Seq percent confirming:41.9355
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAGTTCAGGCGCATCAGG
Suggested primer 2:AACTTCACGTCCAGCCTCAG