Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.041001 |
Chromosome: | chromosome 1 |
Location: | 7442791 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g052550 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCCCGCCACCGCCAGATGCCGCCCCTCCACCTGCTCCTCTGCCTGC |
Internal bar code: | ACTCTAAGAGTCCTGATTGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1959 |
LEAP-Seq percent confirming: | 61.1111 |
LEAP-Seq n confirming: | 11 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTTTGTGATAGGGTGGCT |
Suggested primer 2: | TGAGCAGTCCCTACTCACGA |