| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.041003 |
| Chromosome: | chromosome 17 |
| Location: | 3053285 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720150 | (1 of 1) IPR000104//IPR008752 - Antifreeze protein, type I // Peptidase M11, gametolysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACACAACCTTGCGCTTCAGGAACTTGAGGAGCTGTTGTTTGAGCCGT |
| Internal bar code: | GAGAAGAACACGAGCAGATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1943 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGCTACCAGACACCCAG |
| Suggested primer 2: | GTCGTTCACGGACTTCCCAT |