Insertion junction: CLIP2.041008_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.041008
Chromosome:chromosome 13
Location:2629101
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581050 GT90F46,GT90-46 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 46 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGAGATGACCCGCACGAGCGGGTGCCGCCACAGCCTTCGGCACCACAG
Internal bar code:AATGGTGACTCCGGTTGAGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2134
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTGGCGAGGAACAGGAA
Suggested primer 2:CGTACGGTCCCTGGTTTCAA