Insertion junction: CLIP2.041019_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.041019
Chromosome:chromosome 16
Location:5036080
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686000 SRS15 (1 of 1) K13096 - splicing factor 4 (SF4); putative RNA-binding protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCGCGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGAGTGTG
Internal bar code:AACATAGGTGGTGTAGACGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:548
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AACAAAGATTTGCACCCCGC
Suggested primer 2:CACACACATACACACGCACG