Insertion junction: CLIP2.041033_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.041033
Chromosome:chromosome 4
Location:1757596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g211950 ELG32 Exostosin-like glycosyltransferase 32; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCCACTTACCCCCACTTACCCCAACCCGGGCTGCATCCCACGGACGG
Internal bar code:CTAGACGCTATAGGTGTGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:685
LEAP-Seq percent confirming:72.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGGGCTTGTACCTGGAT
Suggested primer 2:GGCCCTCAGCTCTGTTCAAT