| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041035 |
| Chromosome: | chromosome 12 |
| Location: | 4382692 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g519550 | OPR53 | OctotricoPeptide Repeat protein 53 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTGCTGCTGCCGGCACTGAGCGCCGCCGGCGCGCTCGGCGCCGCG |
| Internal bar code: | GTTGACTGAGTATGCTCTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3356 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATCCCCACGTCCAACAG |
| Suggested primer 2: | GGGGTAGTGCCGAAGATGAG |