| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041049 |
| Chromosome: | chromosome 5 |
| Location: | 1368770 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234636 | PFLAE,PFA1,PFLA1 | Pyruvate formate lyase activating enzyme 1; (1 of 1) 1.97.1.4 - [Formate-C-acetyltransferase]-activating enzyme / S-adenosyl-L-methionine-cleaving) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGCGAGGGTGGGGGTGCGCCCATCTACCACGCACGCCCTGTGGCCT |
| Internal bar code: | GTCATCCATTGATTGAGTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTTTGTCCCCGCTTCAC |
| Suggested primer 2: | ACGCCCCAAATAATCTGCCA |