Insertion junction: CLIP2.041054_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.041054
Chromosome:chromosome 16
Location:2469530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g660350 (1 of 1) K15363 - fanconi-associated nuclease 1 (FAN1, MTMR15) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACATCGCAAGGTGCGTGGGCGCGCGTGTAAACGCGCGCGTCTGCGTG
Internal bar code:TATAATGTTGGATGCCTACCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1890
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTCAGGGCATGAAGAGT
Suggested primer 2:AAAGTGAAGGTAGGGCGGTG