| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041071 |
| Chromosome: | chromosome 2 |
| Location: | 7784409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146550 | (1 of 1) PF14251 - Domain of unknown function (DUF4346) (DUF4346) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGATGAGGCAATGCCGCGTGGCAGGGGTGGTTGGGTTGCTTGTGGCTT |
| Internal bar code: | GTACTAATTTAGCGTCAAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3937 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGCTTTTGTCCTCCCTG |
| Suggested primer 2: | GATAAGCTCTCCTGGCGACC |