| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041072 |
| Chromosome: | chromosome 7 |
| Location: | 6064485 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355250 | WEE1 | CDK inhibitory kinase; (1 of 1) K06632 - wee1-like protein kinase (WEE1) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCACCTTCAGCGGCCGTGGCCGCTTCATTGCGCGGGGCGAGGGTG |
| Internal bar code: | TTTTCGAGACTACAGAGCAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2597 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAATCTCGGCGACATCTCG |
| Suggested primer 2: | AGAGCAACCGTCACTCACAG |