| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.041078 |
| Chromosome: | chromosome 3 |
| Location: | 5972543 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189200 | (1 of 1) PTHR13192//PTHR13192:SF3 - MY011 PROTEIN // METHYLMALONIC ACIDURIA AND HOMOCYSTINURIA TYPE D PROTEIN, MITOCHONDRIAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGGCGCAGCCCTCCGACGCCGCTGCCTGCCTGCGCCGCCTCCCCCA |
| Internal bar code: | GGGCATCGTGGCGTCATATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCTGTTCCTGTAAAGGG |
| Suggested primer 2: | CCTGCACACACTTCAAACGG |