Insertion junction: CLIP2.041101_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041101
Chromosome:chromosome 11
Location:1203666
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467687 (1 of 1) 1.1.1.348 - Vestitone reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGACGACATGGCGCGCCGGCAATCGCTTGCGAGGACACGAGTTCGTGT
Internal bar code:CTTCTGGTTGCGGGTTAGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2790
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCTAAACGCTTCCGCTG
Suggested primer 2:CTTCTGGGTTGGCAAATGGC