Insertion junction: CLIP2.041106_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041106
Chromosome:chromosome 9
Location:6276766
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g413900 GT90F18,GT90-18 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 18 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCTGTTTCACTCCTCTTATTGGCATCATATCCGACCACTACAGACGCG
Internal bar code:TTCAGTAGGGGGGACGATAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5498
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGACACGCAGAAACGAAC
Suggested primer 2:ACGCCAGCATCCCCAATAAA