| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041111 |
| Chromosome: | chromosome 6 |
| Location: | 3239213 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277050 | (1 of 1) K14856 - protein SDA1 (SDA1, SDAD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCATGGCCTTGCGCCGCTGCTTCTCGCGCTCCGACAGACCCGCCGTC |
| Internal bar code: | GCCGGATTGAGCAGGCTCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 30.7692 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAAACACATCGCCACCC |
| Suggested primer 2: | TCAAGCACTCGACTGAGCTG |