Insertion junction: CLIP2.041114_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041114
Chromosome:chromosome 1
Location:3892492
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025600 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCAGGAGCGTGTGCGCCCCACATTCGTGGCGCTGAAGCACAAGGCGC
Internal bar code:CACGCTTCATGGAAGCTCCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1832
LEAP-Seq percent confirming:97.2222
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CGATAGCAGGAAAGGGCGAT
Suggested primer 2:ACCAGTACATTGCGGGTCTG